Statistical Optimization of the production of protease from Bacillus sp. MSK-01 in submerged fermentation

Kulwant Kaur
Department of Biotechnology, chandigarh College of Technology, CGC, Landran, Mohali
Sonica Sondhi
Department of Biotechnology, Chandigarh College of Technology, CGC, Landran, Mohali -140307
Palki Sahib Kaur
Department of Biotechnology, chandigarh College of Technology, CGC, Landran, Mohali

DOI:https://doi.org/10.5912/jcb810

Abstract:

Protease has been in increasing demand in industries due to its hydrolytic nature. In industries, high yield of enzyme is required to meet the industrial need at a relatively cheaper cost. In the present study, the protease from Bacillus sp. MSK-01 was produced in large quantity by submerged fermentation. Statistical techniques including Plackett-Burman and Response surface methodology are useful tools for optimizing many parameters at a time and are used for increasing the protease production from Bacillus sp. MSK-01. 19 different parameters were chosen, out of which 15 factors had positive effect on protease yield. Four maximum influencing factors were peptone, magnesium sulphate, skim milk powder and casein were chosen to further increase the protease yield. 397.3 IU ml^-1 of enzyme yield was obtained under optimized conditions which lead to 198 fold increase in the yield of protease from unoptimized condition.

 

Keywords:Bacillus sp. MSK-01 ,Protease ,Submerged fermentation ,Response surface methodology ,en ,