Industrial Application of Biological Self-healing Concrete: Challenges and Economical Feasibility
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Self-healing concrete has been scrutinized by several researchers and some industrial concrete producers in relation to the remediation of the occurrence of micro-cracks. Such cracks are a quite well known problem that can lead to corrosion of the steel reinforcement and thus to the possible failure of the entire concrete structure. The need to repair these cracks as soon as possible leads to maintenance costs which can be of the order of €130 (direct costs) per m3 of concrete. Recent scientific studies indicate that a Microbial Induced Carbonate Precipitation (MICP), using microbial spores as active agent, can be an alternative for the actual repair methods. However, the production of bacterial spores is yet imposing considerable costs. According to some concrete producers they would be willing to pay about €15 to €20 per m3 of concrete for a bio-based self-healing product. However, the actual cost of spores production and encapsulation represent a total cost which is orders of magnitude higher. This article analyzes the costs for the biological self-healing in concrete and evaluates the industrial challenges it faces. There is an urgent need to develop the production of a bio-additive at much lower costs to make the biological self-healing industrial applicable. Axenic production and a possible non-axenic process to obtain ureolytic spores were analyzed and the costs calculations are presented in this paper.
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Cement Trust. What is the development impact of concrete?, Retrieved 10 January 2013 (

NRMCA – National Ready Mixed Concrete Association. Ready mixed concrete production statistics. Retrieved 10 January 2013 (

ACI Committee 224 (2007). Causes, evaluation and repair of cracks in concrete structures. ACI 224.1R-07. American Concrete Institute, Michigan (

Mihashi, H., Nishiwaki, T. (2012). Development of engineered self-healing and self-repairing concrete-state-of-the-art report. Journal of Advanced Concrete Technology, 10, 170-184 (

Van Tittelboom, K., De Belie, N., De Muynck, W., Verstraete, W., (2010). Use of bacteria to repair cracks in concrete. Cement and Concrete Research, 40, 157-166 (

Malinskii, Y. M., Prokopenko, V. V., Ivanova, N. A., Kargin, V. A., (1970). Investigation of self-healing of cracks in polymers. Polymer Mechanics, 6, 240-244 (

White, S. R., Sottos, N., R., Geubelle, P. H., Moore, J. S., Kessler, M. R., Sriram, S. R., Brown, E. N., Viswanathan, S., (2001). Autonomic healing of polymer composites. Nature, 409, 794-797 (

Igarashi, S., Kunieda, M., Nishiwaki, T., (2009). Research activity of JCI technical committee TC-075B: Autogenous healing in cementitious materials. Proceedings of 4th International Conference on Construction Materials: Performance, Innovations and Structural Implications, 89-96 (

De Rooij, M. R., Schlangen, E. (2011). Self-healing phenomena in cement-based materials. Draft of State-of-the-Art Report of RILEM Technical Committee 221-SHC (

De Muynck, W., Cox, K., De Belie, N., Verstraete, W., (2008). Bacterial carbonate precipitation as an alternative surface treatment for concrete. Construction and Building Materials, 22, 875-885 (

Gollapudi, U. K., Knutson, C. L., Bang, S. S., Islam, M. R., (1995). A new method for controlling leaching through permeable channels. Chemosphere, 30, 695-705 (

Wang, J., Van Tittelboom, K., De Belie, N., Verstraete, W., (2010). Potential of applying bacteria to heal cracks in concrete. Proceedings of the second international conference on sustainable construction materials and technologies. Italy: Ancona, 1807-1818 (

Wang, J., De Belie, N., Verstraete, W., (2011). Self-healing concrete by use of diatomaceous earth immobilized bacteria. 3rd International Conference on Self-healing materials, Bath, 27-29 June 2011 (

Wang, J., De Belie, N., Verstraete, W., (2011). A microbial based system developed for self-healing concrete cracks. 7th International Symposium on Cement Based Materials for a sustainable Agriculture (CSA), Quebec, 18-21 September, 2011 (

Wang, J., Van Tittelboom, K., De Belie, N., Verstraete, W., (2012). Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Construction and building materials, 26, 532-540 (

Wang, J., De Belie, N., Verstraete, W., (2012). Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. Journal of Industrial Microbiology and Biotechnology, 39, 567-577 (

Dick, J., De Windt, W., De Graef, B., Saveyn, H., Van der Meeren, P., De Belie, N., Verstraete, W., (2006). Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegradation, 17, 357-367 (

Hammes, F., Verstraete, W. (2002). Key roles of pH and calcium metabolism in microbial carbonate precipitation. Environmental Science and Biotechnology, 1, 3-7 (

Hammes, F., Seka, A., de Knijf, S., Verstraete, W., (2003). A novel approach to calcium removal from calcium-rich industrial wastewater. Water Research, 37, 699-704 (

Hammes, F., Boon, N., de Villiers, J., Verstraete, W., Siciliano, S. D., (2003). Strain-specific ureolytic microbial calcium carbonate precipitation. Applied and Environmental Microbiology, 69, 4901-4909 (

Qian, C., Wang, J., Wang, R., Cheng, L., (2009). Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii. Materials Science and Engineering C, 29, 1273-1280 (

Wang, J., (2012). Self-healing concrete by means of immobilized carbonate precipitating bacteria. PhD Thesis. ISBN 978-90-8578-583-5 (

European Committee for Standardization. (2000). European Standard EN206-1. Concrete – Part 1: Specification, performance, production and conformity (

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