Leveraging CRISPR-Cas9 Technology for Biopharmaceutical Development: Challenges and Market Opportunities

Abstract

CRISPR-Cas9 is a revolutionary technique for making precise and effective changes to genetic material. This article gives a detailed review of CRISPR-Cas9 technology and its use in genome editing. The fundamental concepts of CRISPR-Cas9 technology are first described, including how the system targets specific DNA regions in the genome via the Cas9 nuclease, which generates targeted double-strand breaks using a single guide RNA (sgRNA). We provide in-depth assessments of CRISPR-Cas9 technology and its applications in environmental science, biotechnology, bioinformatics, nanotechnology, fisheries, and agriculture. We also talk about its potential, fresh studies, and the ethical quandaries and arguments surrounding its implementation. This work may pave the way for future improvements and appropriate deployments of CRISPR-Cas9 technology by increasing our understanding of the technology and its applications in a range of fields. CRISPR-Cas9 technology, which allows for precise and efficient genetic modification, has altered the area of genome editing. CRISPR-Cas9 is a very effective gene-editing tool that takes use of the bacterial immune system's ability to target and destroy certain DNA regions. A single guide RNA (sgRNA) is a small RNA molecule that initiates a double-strand break and directs the Cas9 nuclease to the target DNA region. Cellular repair processes can then heal the breach, allowing the necessary genetic alterations to be introduced. The simplicity of usage, low cost, and high efficiency of CRISPR-Cas9 have transformed the field of genetic engineering.