Statistical Optimization of the Production of alpha-Amylase from Bacillus licheniformis MTCC 1483 Using Paddy Straw as Substrate

Issue JCB, Volume 23, Number 2, 2017 

Palki Sahib Kaur
Punjab Technical University
Sukhjeet Kaur
Hardish Kaur
Sonica Sondhi

DOI:https://doi.org/10.5912/jcb784


Abstract:

?-Amylase has been in increasing demand in industries due to its hydrolytic nature. Solid state fermentation (SSF) is a cost effective method for increasing the enzyme production. In the present study, amylase from Bacillus licheniformis MTCC 1483 was produced in large quantity by solid state fermentation using paddy straw as substrate. Response surface methodology is a useful tool for optimizing many parameters at a time and is used for increasing the amylase production. 8523 IUg-1 of enzyme activity was obtained under optimized conditions which lead to 35 fold increase in the yield of amylase from unoptimized condition.

Keywords:Bacillus licheniformis ,Amylase ,Solid state fermentation ,Response surface methodology ,Punjab Technical University ,en ,

Download PDF